Journal: Animals : an Open Access Journal from MDPI
Article Title: Combining Transcriptomics and Proteomics to Screen Candidate Genes Related to Bovine Birth Weight
doi: 10.3390/ani14182751
Figure Lengend Snippet: Data validation through qRT-PCR and Western blot. ( A ) Comparison of the 16 mRNAs using qRT-PCR and RNA-Seq, where the orange reflects the qRT-PCR outcomes and the blue indicates the RNA-Seq outcomes. The x -axis represents the names of the genes, and the y -axis represents the log2(FC). ( B – D ) The Western blot results analysis of PAK1 and ITGA3 in different groups. Values are expressed as means ± SEM ( n = 3). The left y -axis is the protein expression, the right y -axis is the FPKM value, and the x -axis is the grouping. ACSL1 , acyl-CoA synthetase long-chain family member 1; ELOVL5 , ELOVL fatty acid elongase 5; ELOVL7 , ELOVL fatty acid elongase 7; IQGAP2 , IQ motif containing GTPase-activating protein 2; ITGA3 , integrin subunit alpha 3; PAK1 , p21 (RAC1)-activated kinase 1; CYP11A1 , cytochrome P450 family 11 subfamily A member 1; CYP17A1 , cytochrome P450 family 17 subfamily A member 1; PDLIM1 , PDZ and LIM domain 1; TMAB4X , thymosin beta 4 X-linked; COL14A1 , collagen type XIV alpha 1 chain; SERPINH1 , serpin family H member 1; CD81 , CD81 molecule; SLC23A2 , solute carrier family 23 member 2; TAGLN , transgelin; S100A13 , S100 calcium-binding protein A13.
Article Snippet: The candidate placental protein marker P21-activated kinase 1 (PAK1) (1:1000, item No. A25545) and Integrin α3β1 (ITGA3) (1:1000, item No. A17502), purchased from ABclonal Technology Co. Ltd., were compared with the GAPDH expression using Image J_v1.8.4.
Techniques: Quantitative RT-PCR, Western Blot, Comparison, RNA Sequencing Assay, Expressing, Binding Assay